It’s been a while since I’ve blogged about my own work.  I thought I’ve give an update.

I have the chaperone of the CS1 pathway cloned onto a vector and in a host strain.  But for some reason, I haven’t been able to induce  expression to high enough levels to be useful for an overlay assay, in the periplasm prep.

In english, Gram-negative Enterotoxigenic E. coli  (ETEC) have two membranes.  I strip the outher membrane off and collect the proteins in the periplasm space.  But, for some reason, the protein I’m trying to collect, which chaperones other CS1 pilus proteins between the two membranes, is not to be found in the periplasm.

Therefore, I’m going to take the gene out of the plasmid vector its currently in and try putting it into a different one.  Wish me luck.

This entry was posted on Monday, June 9th, 2008 at 1:57 pm and is filed under microbiology, musings, my research. You can follow any responses to this entry through the RSS 2.0 feed. You can leave a response, or trackback from your own site.