Here’s another update on my research.  My boss decided that I should stop playing around with bacterial cloning and actually get to the meat of the experiment.  I’m finally doing those overlay assays I’ve been talking about for the last year.

In a nutshell, it’s running proteins on a gel, overlaying other proteins on top of them, and seeing what sticks.  It’ll show us what proteins in the CS1 pilus system bind together.  The purpose it to determine the binding order and assembly mechanism.  Wish me luck.

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